Modifying Enzymes
New England Biolabs, Inc. Antarctic Thermolabile UDG – 100 units
Antarctic Thermolabile UDG (Uracil- DNA Glycosylase) catalyzes the release of free uracil from uracil-containing single-stranded or double-stranded DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperature and high pH. This enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50C.
Zymo Research Corporation dsDNA Shearase™ Plus 50 U (1 U/ul) + DCC-5 (D4013) '**'
dsDNA Shearase™ Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5-phosphate and 3-hydroxyl termini. It has a particularly strong preference for double-stranded DNA (dsDNA) and generates random-ended DNA fragments of the desired size in a single step. Sequencing data demonstrates that dsDNA Shearase™ Plus does not introduce any detectable bias in the sequencing library preparation. This enzyme is compatible with low volume inputs thus minimizing sample loss. Digested DNA is easily purified in 10 l with recommended DNA Clean & Concentrator™ technology making it ideal for use in end modification (linker & adapter) procedures and other applications.
Sigma Aldrich Fine Chemicals Biosciences Lipase immoblilized on Imm50G
LIPASE Lipase immoblilized on Imm50G
Cayman Chemical N-Oleoyl GlycIn 100mg
An endogenous lipoamino acid; increases triglyceride levels in 3T3-L1 adipocytes, an effect that can be reversed by SR141716; selectively induces the expression of mRNA encoding the CB1 receptor over mRNA encoding the CB2 receptor in the same cells at 50 µM; potentiates glycine-induced chloride currents in Xenopus oocytes expressing α1-, α1β-, α2-, α2β-, or α3 subunit-containing glycine receptors but not those expressing α3β subunit-containing glycine receptors at 1 µM; increases c-Fos protein levels, intracellular calcium levels, and AgRP secretion in mHypoE-38 cells, effects that can be reversed by AM251; reduces binge-like ethanol intake in alcohol-preferring mice without affecting total fluid intake and does not affect sucrose intake in the sucrose preference test in mice at 60 mg/kg; reduces balance impairments, thermal hyperalgesia, mechanical allodynia, aggressiveness, and immobility time in the tail suspension test in a mouse model of traumatic brain injury at 50 mg/kg
Sigma Aldrich Fine Chemicals Biosciences alpha Amylase from Bacillu50G
We are committed to bringing youAGreenerAAlternative Products which adhere to one or more of The 12 Principles ofAGreenerAChemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. for more information see the article in biofiles.
![Research Products International Corp Cellulase RS [Onozuka RS], 5 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C32400-5.0.JPG-250.jpg)
Research Products International Corp Cellulase RS [Onozuka RS], 5 Grams
From mutant Trichoderma viride derived from the parent strain for Cellulase Onozuka R-10. Cellulase RS contains a high activity of decomposing natural celluloses. Can be used to obtain protoplasts and dissolve cell walls in a wide range of plants.
LGC GENOMICS LLC (Lucigen) RNase A, 2 mL @ 5 mg/mLEfficiently degrade unwanted RNA in DNA preparations and other samples.
This reagent is designed for use with the MasterPure™ kit protocols only.
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 50MG
Catalase from bovine liver aqueous solution, >=30,000 units/mg protein | 9001-05-2 | MFCD00081483 | 50MG
Supelco Inc HYALURONIDASE TYPE 1V-S
Hyaluronidase, Type IV-S, lyophilized powder (essentially salt-free), 750-3000A units/mg solid
New England Biolabs, Inc. Cre Recombinase – 50 units
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused. DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences.
New England Biolabs, Inc. Taq DNA Ligase Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Taq DNA Ligase. Taq DNA Ligase requires NAD+ as a cofactor which is incorporated into the buffer. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. Fpg – 2500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 200 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
DESERT BIOLOGICALS RESEARCH Macerozyme R10
Standardized at 3000 maceration units/gram.The activity is based on measuring the volume of single cells released from potato tuber disks. pH: 5.0-6.0